Ahmed Elshobaky

High-Resolution Biomarker Detection Using Multiplex Digital PCR: Evidence from Clinical Research

Biomarker-based patient profiling in liquid biopsies using digital PCR (dPCR) is redefining the future of healthcare. This presentation will explore how dPCR enhances biomarker detection across diverse disease areas, supported by scientific case studies. The ability to detect low levels of cell-free DNA (cfDNA) and overcome PCR inhibitors in heterogeneous samples underscores the need for optimal diagnostic and monitoring techniques. dPCR provides an ideal solution for precise therapy evaluation, offering superior specificity, sensitivity, and reproducibility compared to other technologies—particularly for assessing Minimal Residual Disease (MRD). Real-world examples from clinical trials will illustrate the growing clinical utility of ctDNA-MRD testing in guiding therapeutic decisions and improving patient outcomes.